ABSTRACT
Shelf life determination of herbal medicines is of paramount importance as it relates to activity of constituents of theproduct. This work sought to determine shelf life of four herbal products (Nibima, Asena, Lippia tea, and NPK 500capsules). The method involved the determination of marker content of products (three batches each) at time points(0, 3, 6, 9, 12, and 18 months) at storage temperature and humidity of 30°C ± 2°C/70% RH ± 5% RH using highperformance liquid chromatography (HPLC) analyses. Batch blending was employed for preparation of referencesamples of products. In UV analyses, λmax of 289, 291, 327, and 289 nm were obtained from spectra for (Nibima,Asena, Lippia tea, and NPK 500 capsules, respectively. A common 230 nm UV marker was observed for all theproducts. Optimized HPLC conditions were developed for products using methanol: water: 0.1%v/v acetic acidsystem with mobile phase ratios of 9:0:1 (Nibima), 7:2:1 (Asena), 8:1:1 (Lippia tea), and 90:5:5 (NPK 500 capsules).Wavelength of detection used for HPLC analyses were 283, 290, 332, and 290 nm for Nibima, Asena, Lippia tea, andNPK 500 capsules, respectively. HPLC marker content analyses with time produced shelf life of 23.14, 21.16, 62.97and 32.91 months for Nibima, Asena, Lippia tea, and NPK 500 capsules, respectively. Obtained shelf life indicatesrelative stability of products.
ABSTRACT
A rapid analysis method based on ultraviolet-visual(UV-Vis) spectroscopy, near infrared(NIR) spectroscopy and multivariable data analysis was established for quality evaluation of Shengxuebao Mixture. The contents of eight active ingredients of Shengxuebao Mixture including albiflorin, paeoniflorin, 2, 3, 5, 4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside, specnuezhenide,ecliptasaponin D, emodin, calycosin-7-glucoside and astragaloside Ⅳ were simultaneously detected by using this method. HPLC-UV-MS was used as a reference method for determining the contents of these ingredients. Partial least squares(PLS) analysis was implemented as a linear method for multivariate models calibrated between UV spectrum/NIR spectrum and contents of 8 ingredients. Finally, the performance of the model was evaluated by 24 batches of test samples. The results showed that both UV-Vis and NIR models gave a good calibration ability with an R~2 value above 0.9, and the prediction ability was also satisfactory, with an R~2 value higher than 0.83 for UV-Vis model and higher than 0.79 for NIR model. The overall results demonstrate that the established method is accurate, robust and fast, therefore, it can be used for rapid quality evaluation of Shengxuebao Mixture.
Subject(s)
Calibration , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Least-Squares Analysis , Mass Spectrometry , Spectroscopy, Near-InfraredABSTRACT
This study describes the formulation of immediate release Ketorolac tromethamine (KT) 10-mg tablet by directcompression method; evaluation of their compliance to various Pharmacopoeial quality parameters, i.e., weightvariation, friability, hardness, thickness, moisture content, disintegration, assay, and dissolution; and their comparisonwith marketed brands for determination of pharmaceutical equivalency. Five formulations of KT were prepared(coded as FKT1, FKT2, FKT3, FKT4, and FKT5) by direct compression method using different superdisintegrants.Micrometric properties of the mixtures of the drug and the excipients prepared for formulation were evaluated. Qualityevaluation of the five different formulations and randomly selected four different brands of KT 10-mg tablets purchasedfrom the local market (coded as LKT1, MKT2, MKT3, and SKT4) were performed according to Pharmacopoeia. Theresults were obtained by UV-Vis spectrophotometer and all the dissolution profiles were characterized by the zeroorder kinetics. All the brands of KT and developed formulations met the official specification except SKT4 whichshowed excessive moisture content of 7.18%. None of the tested brands of KT were found to be pharmaceuticallyequivalent, whereas two developed formulation were pharmaceutically equivalent with the in house benchmark(MKT2) from which their interchangeability can be suggested.
ABSTRACT
ABSTRACTThe aim of this study was to optimize the biosynthesis of silver nanoparticles using leaves ofAegle marmelos as the primary source. The optimal reaction medium comprised 2:1 concentration of leaf extract and 6mM concentration of silver nitrate solution (pH 7. The biosynthesized silver nanoparticles were confirmed by UV-Vis spectroscopy at 420 nm, XRD and FTIR analysis. The antimicrobial properties of silver nanoparticles were confirmed withBacillus subtilis andPseudomonas aeruginosa.
ABSTRACT
Objective: To prepare rutin-Cu2+ complex imprinted polymer and study its adsorption ability. Methods: The polymer was prepared in mixed poler solvent, using α-methacrylic acid as functional monomer. The structure and preparation mechanism were studied by UV-vis spectroscopy and IR spectroscopy. Results: The polymer and rutin-Cu2+ complex were combined by coordinate bond and intermolecular force, the polymer had remarkably specific adsorption to complex of rutin-Cu2+. Conclusion: Rutin-Cu2+ complex molecularly imprinted polymer has great adsorption and specific identification to rutin-Cu2+, and can be used in extraction and separation of Chinese medicine effective constituent rutin.